Article

NEXT-FRET: A solution-based smFRET platform to resolve folding intermediates under native conditions

2025-08-01

Abstract excerpt

Folding intermediates are promising therapeutic targets in protein misfolding, bacterial virulence, and drug discovery. Yet, directly observing these transient, non-equilibrium states under physiological conditions remains challenging. Here, we introduce NEXT-FRET (Non-Equilibrium miXTure modeling of smFRET), a solution-based single-molecule FRET (smFRET) platform integrating accessible instrumentation and a time-...

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Literature Corpus work: 7916fd0d-1ce6-5d31-ac90-8d8b4cb1577e
DOI: 10.1101/2025.07.30.666321

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