Structural basis for alternative 3′ splice site selection in the human spliceosome active center

Abstract excerpt

Accurate alternative splicing requires discrimination between adjacent 3′ splice sites (3′-ss) during catalysis and is disrupted by pathogenic AG-gain mutations that create competing 3′-ss. Here, we present cryo-EM structures of human spliceosomes assembled on native-sequence pre-mRNAs, revealing how the catalytic core controls alternative 3′-ss selection. SDE2 is a previously unrecognized active-center component...

Topics

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• Catalytic Domain
• Mutation
• Neoplasms
• Phosphoproteins
• RNA Precursors
• RNA, Small Nuclear
• RNA Splicing Factors
• Spliceosomes

Identifiers and source

Literature Corpus work

c4961eb5-e74d-547a-ae1c-21795addf65b

DOI

10.64898/2026.08.05.741260