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Article

Preparation of fluorescent pre-mRNA substrates for an smFRET study of pre-mRNA splicing in yeast.

Methods in enzymology - 1 Jan 2010

Abelson John, Hadjivassiliou Haralambos, Guthrie Christine

Abstract excerpt

The spliceosome is a complex small nuclear (sn)RNA-protein machine that removes introns from pre-mRNAs via two successive phosphoryl transfer reactions. For each splicing event, the spliceosome is assembled de novo on a pre-mRNA substrate and a complex series of assembly steps leads to the active...

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PMID: 20580958
DOI: 10.1016/S0076-6879(10)72017-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-20580958-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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