Article
Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity.
Journal of biotechnology - 10 Oct 2015
Anderson Emily M, Haupt Amanda, Schiel John A, Chou Eldon, Machado Hidevaldo B, Strezoska Žaklina, Lenger Steve, McClelland Shawn, Birmingham Amanda, Vermeulen Annaleen, Smith Anja van Brabant
Abstract excerpt
The discovery that the bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) acquired immune system can be utilized to create double-strand breaks (DSBs) in eukaryotic genomes has resulted in the ability to create genomic changes more easily than with other genome engineering techniques. While there is significant potential for the CRISPR-Cas9 system to...
Topics
- Base Pair Mismatch
- Base Sequence
- CRISPR-Cas Systems
- Cell Line, Tumor
- Gene Targeting
- Genes, Reporter
- Green Fluorescent Proteins
- HEK293 Cells
- Humans
- Molecular Sequence Data