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Article

CRISPR-Cas effector specificity and cleavage site determine phage escape outcomes.

PLoS biology - 1 Apr 2023

Schelling Michael A, Nguyen Giang T, Sashital Dipali G

Abstract excerpt

CRISPR-mediated interference relies on complementarity between a guiding CRISPR RNA (crRNA) and target nucleic acids to provide defense against bacteriophage. Phages escape CRISPR-based immunity mainly through mutations in the protospacer adjacent motif (PAM) and seed regions. However, previous specificity studies of Cas effectors, including the class 2 endonuclease Cas12a, have revealed a high degree of...

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Identifiers

PMID: 37058530
DOI: 10.1371/journal.pbio.3002065
PMCID: PMC10132644

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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