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Article

Determinants for Efficient Editing with Cas9-Mediated Recombineering in Escherichia coli.

ACS synthetic biology - 15 May 2020

Choudhury Alaksh, Fankhauser Reilly G, Freed Emily F, Oh Eun Joong, Morgenthaler Andrew B, Bassalo Marcelo C, Copley Shelley D, Kaar Joel L, Gill Ryan T

Abstract excerpt

In E. coli, editing efficiency with Cas9-mediated recombineering varies across targets due to differences in the level of Cas9:gRNA-mediated DNA double-strand break (DSB)-induced cell death. We found that editing efficiency with the same gRNA and repair template can also change with target position, cas9 promoter strength, and growth conditions. Incomplete editing, off-target activity, nontargeted mutations, and...

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Identifiers

PMID: 32298586
DOI: 10.1021/acssynbio.9b00440

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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