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Article

CRISPRseek: a bioconductor package to identify target-specific guide RNAs for CRISPR-Cas9 genome-editing systems.

PloS one - 1 Jan 2014

Zhu Lihua J, Holmes Benjamin R, Aronin Neil, Brodsky Michael H

Abstract excerpt

CRISPR-Cas systems are a diverse family of RNA-protein complexes in bacteria that target foreign DNA sequences for cleavage. Derivatives of these complexes have been engineered to cleave specific target sequences depending on the sequence of a CRISPR-derived guide RNA (gRNA) and the source of the Cas9 protein. Important considerations for the design of gRNAs are to maximize aimed activity at the desired target...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 25247697
DOI: 10.1371/journal.pone.0108424
PMCID: PMC4172692

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Source receipt: pubmed, retrieved 7 Sept 2026.

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