Article
Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo.
Nucleic acids research - 20 Jun 2016
Fu Becky X H, St Onge Robert P, Fire Andrew Z, Smith Justin D
Abstract excerpt
Cas9, a CRISPR-associated RNA-guided nuclease, has been rapidly adopted as a tool for biochemical and genetic manipulation of DNA. Although complexes between Cas9 and guide RNAs (gRNAs) offer remarkable specificity and versatility for genome manipulation, mis-targeted events occur. To extend the understanding of gRNA::target homology requirements, we compared mutational tolerance for a set of Cas9::gRNA complexes...
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