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CRISPR-Cas9-Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library.

Cell stem cell - 6 Aug 2015

Zhong Cuiqing, Yin Qi, Xie Zhenfei, Bai Meizhu, Dong Rui, Tang Wei, Xing Yu-Hang, Zhang Hongling, Yang Suming, Chen Ling-Ling, Bartolomei Marisa S, Ferguson-Smith Anne, Li Dangsheng, Yang Li, Wu Yuxuan, Li Jinsong

Abstract excerpt

Mouse androgenetic haploid embryonic stem cells (AG-haESCs) can support full-term development of semi-cloned (SC) embryos upon injection into MII oocytes and thus have potential applications in genetic modifications. However, the very low birth rate of SC pups limits practical use of this approach. Here, we show that AG-haESCs carrying deletions in the DMRs (differentially DNA methylated regions) controlling two...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26165924
DOI: 10.1016/j.stem.2015.06.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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