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Protocol for DNA Methylation Editing of Imprinted Loci and Assessment of the Effects.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Sekita Yoichi, Kimura Tohru

Abstract excerpt

In this chapter, we present an experimental protocol to conduct DNA methylation editing experiments, that is, to induce loss or gain of DNA methylation, targeting Dlk1-Dio3 imprinted domain, a well-studied imprinted locus, in ES cells. In this protocol, plasmid vectors expressing the DNA methylation editing tools, combining the CRISPR/dCas9 system and the SunTag system coupled to a DNA methyltransferase or a TET...

Topics

Genomic Imprinting
DNA Methylation
Gene Editing
Animals
CRISPR-Cas Systems
Mice
Genetic Loci
Calcium-Binding Proteins
Iodide Peroxidase
Alleles
Humans

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PMID: 39012595
DOI: 10.1007/978-1-0716-4051-7_8

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Protocol for DNA Methylation Editing of Imprinted Loci and Assessment of the Effects.PMID 39012595Read ↗

Epigenome editing reveals core DNA methylation for imprinting control in the Dlk1-Dio3 imprinted domain.Semantic proximity Read ↗

A bipartite element with allele-specific functions safeguards DNA methylation imprints at the <i>Dlk1-Dio3</i> locusSemantic proximity Read ↗

A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus.Semantic proximity Read ↗

Epigenetic control and genomic imprinting dynamics of the Dlk1-Dio3 domainSemantic proximity Read ↗

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TET3 protects the Dlk1-Dio3 Imprinted Locus from DNA hypomethylation during adult NSC ReprogrammingSemantic proximity Read ↗

Efficient production and transmission of CRISPR/Cas9-mediated mutant alleles at the IG-DMR via generation of mosaic mice using a modified 2CC method.Semantic proximity Read ↗

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The Evolution of the DLK1-DIO3 Imprinted Domain in MammalsSemantic proximity Read ↗

Development of super-specific epigenome editing by targeted allele-specific DNA methylationSemantic proximity Read ↗

Deletion of conserved sequences in IG-DMR at Dlk1-Gtl2 locus suggests their involvement in expression of paternally expressed genes in mice.Semantic proximity Read ↗

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