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Article

An oocyte-specific Cas9-expressing mouse for germline CRISPR/Cas9-mediated genome editing.

Genesis (New York, N.Y. : 2000) - 1 Apr 2024

Lanza Denise G, Mao Jianqiang, Lorenzo Isabel, Liao Lan, Seavitt John R, Ljungberg M Cecilia, Simpson Elizabeth M, DeMayo Francesco J, Heaney Jason D

Abstract excerpt

Cas9 transgenes can be employed for genome editing in mouse zygotes. However, using transgenic instead of exogenous Cas9 to produce gene-edited animals creates unique issues including ill-defined transgene integration sites, the potential for prolonged Cas9 expression in transgenic embryos, and increased genotyping burden. To overcome these issues, we generated mice harboring an oocyte-specific, Gdf9 promoter...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 38523431
DOI: 10.1002/dvg.23589
PMCID: PMC10987075

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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