Article
Targeted Modification of Gene Function Exploiting Homology-Directed Repair of TALEN-Mediated Double-Strand Breaks in Barley.
G3 (Bethesda, Md.) - 6 Jul 2015
Budhagatapalli Nagaveni, Rutten Twan, Gurushidze Maia, Kumlehn Jochen, Hensel Goetz
Abstract excerpt
Transcription activator-like effector nucleases open up new opportunities for targeted mutagenesis in eukaryotic genomes. Similar to zinc-finger nucleases, sequence-specific DNA-binding domains can be fused with effector domains like the nucleolytically active part of FokI to induce double-strand breaks and thereby modify the host genome on a predefined target site via nonhomologous end joining. More...
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