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Article

Site-specific gene targeting using transcription activator-like effector (TALE)-based nuclease in Brassica oleracea.

Journal of integrative plant biology - 1 Nov 2013

Sun Zijian, Li Nianzu, Huang Guodong, Xu Junqiang, Pan Yu, Wang Zhimin, Tang Qinglin, Song Ming, Wang Xiaojia

Abstract excerpt

Site-specific recognition modules with DNA nuclease have tremendous potential as molecular tools for genome targeting. The type III transcription activator-like effectors (TALEs) contain a DNA binding domain consisting of tandem repeats that can be engineered to bind user-defined specific DNA sequences. We demonstrated that customized TALE-based nucleases (TALENs), constructed using a method called "unit...

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PMID: 23870552
DOI: 10.1111/jipb.12091

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