Article
Characteristics of Genome Editing Mutations in Cereal Crops.
Trends in plant science - 1 Jan 2017
Zhu Changfu, Bortesi Luisa, Baysal Can, Twyman Richard M, Fischer Rainer, Capell Teresa, Schillberg Stefan, Christou Paul
Abstract excerpt
Designer nucleases allow the creation of new plant genotypes by introducing precisely-targeted double-strand breaks that are resolved by endogenous repair pathways. The major nuclease technologies are meganucleases, zinc-finger nucleases, transcription activator-like effector nucleases, and the CRISPR/Cas9 system. Each comprises a promiscuous endonuclease guided by protein-DNA or RNA-DNA interactions. A great...
Topics
- CRISPR-Cas Systems
- Crops, Agricultural
- Edible Grain
- Gene Editing
- Genome, Plant
- Mutation