Article

Efficient protein tagging and <i>cis</i> -regulatory element engineering via precise and directional oligonucleotide-based targeted insertion in plants

2023-04-21

Abstract excerpt

Efficient and precise targeted insertion holds great promise but remains challenging in plant genome editing. An efficient NHEJ-mediated targeted insertion method was recently developed by combining CRISPR-Cas9 with phosphorothioate modified double-stranded oligodeoxynucleotides (dsODNs). Yet this approach often led to imprecise insertions with no control over the insertion direction. In this study, we first quant...

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Literature Corpus work: c991c933-9f93-57d1-a442-64fe08060c0f
DOI: 10.1101/2023.04.20.537731

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