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Article

True-breeding targeted gene knock-out in barley using designer TALE-nuclease in haploid cells.

PloS one - 1 Jan 2014

Gurushidze Maia, Hensel Goetz, Hiekel Stefan, Schedel Sindy, Valkov Vladimir, Kumlehn Jochen

Abstract excerpt

Transcription activator-like effector nucleases (TALENs) are customizable fusion proteins able to cleave virtually any genomic DNA sequence of choice, and thereby to generate site-directed genetic modifications in a wide range of cells and organisms. In the present study, we expressed TALENs in pollen-derived, regenerable cells to establish the generation of instantly true-breeding mutant plants. A gfp-specific...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 24643227
DOI: 10.1371/journal.pone.0092046
PMCID: PMC3958423

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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