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Article

Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease.

Genome biology - 30 Nov 2015

Lawrenson Tom, Shorinola Oluwaseyi, Stacey Nicola, Li Chengdao, Østergaard Lars, Patron Nicola, Uauy Cristobal, Harwood Wendy

Abstract excerpt

BACKGROUND: The RNA-guided Cas9 system represents a flexible approach for genome editing in plants. This method can create specific mutations that knock-out or alter target gene function. It provides a valuable tool for plant research and offers opportunities for crop improvement. RESULTS: We investigate the use and target specificity requirements of RNA-guided Cas9 genome editing in barley (Hordeum vulgare) and...

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Identifiers

PMID: 26616834
DOI: 10.1186/s13059-015-0826-7
PMCID: PMC4663725

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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