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Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Yi Li, Taft Joseph M, Li Qing, Gebhard Mark C, Georgiou George, Iverson Brent L

Abstract excerpt

There is significant interest in engineering proteases with desired proteolytic properties. We describe a high-throughput fluorescence-activated cell sorting (FACS) assay for detecting altered proteolytic activity of protease in yeast, at the single cell level. This assay relies on coupling yeast endoplasmic reticulum (ER) retention, yeast surface display, and FACS analysis. The method described here allows...

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PMID: 26060071
DOI: 10.1007/978-1-4939-2748-7_5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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