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Article

Enzyme-substrate co-display on yeast empowers engineering of tobacco etch virus protease activity.

Protein engineering, design & selection : PEDS - 10 Jan 2025

Mikolajczyk Brian M, Golinski Alexander W, Hackel Benjamin J

Abstract excerpt

Engineering improved protease activity using directed evolution is challenged by uncertainty in sequence-function mapping and inefficiency in evaluating activity of candidate mutants. We implemented a generalizable yeast surface display approach that co-displays protease mutants with substrate on the same Aga2 anchor protein. Identification of enhanced activity mutants is enabled by protease cleavage of tethered...

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PMID: 41001844
DOI: 10.1093/protein/gzaf011
PMCID: PMC13010152

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