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PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein Regulators

2025-10-26

Abstract excerpt

Periplasmic display in yeast is a promising but underdeveloped method for screening and studying genetically encoded biomolecules. We present PIONEER, a modular platform that localizes peptides, proteins, and nanobodies to the membrane-proximal periplasmic space of S. cerevisiae , enabling direct interrogation of membrane protein function. By optimizing secretion signals and display scaffolds, PIONEER ensures sta...

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Identifiers and source

Literature Corpus work
74f89ca9-a2dc-543d-b688-fff6ea510750
DOI
10.1101/2025.10.26.684622
Open publication

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PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein RegulatorsDOI 10.1101/2025.10.26.684622
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