Article
A tobacco etch virus protease with increased substrate tolerance at the P1' position.
PloS one - 1 Jan 2013
Renicke Christian, Spadaccini Roberta, Taxis Christof
Abstract excerpt
Site-specific proteases are important tools for in vitro and in vivo cleavage of proteins. They are widely used for diverse applications, like protein purification, assessment of protein-protein interactions or regulation of protein localization, abundance or activity. Here, we report the development of a procedure to select protease variants with altered specificity based on the well-established Saccharomyces...
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