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Article

Direct selection for sequences encoding proteases of known specificity.

Proceedings of the National Academy of Sciences of the United States of America - 15 Jun 1991

Smith T A, Kohorn B D

Abstract excerpt

We have developed a simple genetic selection that could be used to isolate eukaryotic cDNAs encoding proteases that cleave within a defined amino acid sequence. The selection was developed by using the transcription factor GAL4 from Saccharomyces cerevisiae as a selectable marker, a cloned protease from tobacco etch virus (TEV), and an 18-amino acid TEV protease target sequence. In yeast, TEV protease cleaves its...

Topics

Base Sequence
DNA
DNA-Binding Proteins
Endopeptidases
Fungal Proteins
Hydrolysis
Molecular Sequence Data
Phenotype
Plant Viruses
Plasmids
Saccharomyces cerevisiae

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Saccharomyces cerevisiae Proteins

Substrate Specificity

Transcription Factors

Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 2052595
DOI: 10.1073/pnas.88.12.5159
PMCID: PMC51831

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2052595-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Direct selection for sequences encoding proteases of known specificity.PMID 2052595Read ↗

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