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Article

Direct selection for sequences encoding proteases of known specificity.

Proceedings of the National Academy of Sciences of the United States of America - 15 Jun 1991

Smith T A, Kohorn B D

Abstract excerpt

We have developed a simple genetic selection that could be used to isolate eukaryotic cDNAs encoding proteases that cleave within a defined amino acid sequence. The selection was developed by using the transcription factor GAL4 from Saccharomyces cerevisiae as a selectable marker, a cloned protease from tobacco etch virus (TEV), and an 18-amino acid TEV protease target sequence. In yeast, TEV protease cleaves its...

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Identifiers

PMID: 2052595
DOI: 10.1073/pnas.88.12.5159
PMCID: PMC51831

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Direct selection for sequences encoding proteases of known specificity.PMID 2052595Read ↗

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Enzyme-substrate co-display on yeast empowers engineering of tobacco etch virus protease activity.Semantic proximity Read ↗

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An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents.Semantic proximity Read ↗

High-throughput Activity Reprogramming of Proteases (HARP)Semantic proximity Read ↗

Efficient protein depletion by genetically controlled deprotection of a dormant N-degron.Semantic proximity Read ↗

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