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Article

Very high resolution single pass HLA genotyping using amplicon sequencing on the 454 next generation DNA sequencers: Comparison with Sanger sequencing.

Human immunology - 1 Dec 2015

Yamamoto F, Höglund B, Fernandez-Vina M, Tyan D, Rastrou M, Williams T, Moonsamy P, Goodridge D, Anderson M, Erlich H A, Holcomb C L

Abstract excerpt

Compared to Sanger sequencing, next-generation sequencing offers advantages for high resolution HLA genotyping including increased throughput, lower cost, and reduced genotype ambiguity. Here we describe an enhancement of the Roche 454 GS GType HLA genotyping assay to provide very high resolution (VHR) typing, by the addition of 8 primer pairs to the original 14, to genotype 11 HLA loci. These additional...

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PMID: 26037172
DOI: 10.1016/j.humimm.2015.05.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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