Article
Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples.
PloS one - 1 Jan 2016
Yin Yuxin, Lan James H, Nguyen David, Valenzuela Nicole, Takemura Ping, Bolon Yung-Tsi, Springer Brianna, Saito Katsuyuki, Zheng Ying, Hague Tim, Pasztor Agnes, Horvath Gyorgy, Rigo Krisztina, Reed Elaine F, Zhang Qiuheng
Abstract excerpt
BACKGROUND: Unambiguous HLA typing is important in hematopoietic stem cell transplantation (HSCT), HLA disease association studies, and solid organ transplantation. However, current molecular typing methods only interrogate the antigen recognition site (ARS) of HLA genes, resulting in many cis-trans ambiguities that require additional typing methods to resolve. Here we report high-resolution HLA typing of 10,063...
Topics
- Alleles
- Exons
- Gene Frequency
- Genotype
- HLA Antigens
- High-Throughput Nucleotide Sequencing
- Histocompatibility Testing
- Humans
- Mouth Mucosa
- Multiplex Polymerase Chain Reaction