Article
High-throughput next-generation sequencing to genotype six classical HLA loci from 96 donors in a single MiSeq run.
HLA - 1 Nov 2017
Ehrenberg P K, Geretz A, Sindhu R K, Vayntrub T, Fernández Viña M A, Apps R, Michael N L, Thomas R
Abstract excerpt
Next generation sequencing (NGS) methods have been established as an efficient approach for HLA typing because unlike traditional Sanger sequencing, they provide unambiguous results at a reasonable cost. We previously developed a multi-locus index method to genotype four HLA loci (A, B, C, and DRB1) on the Illumina MiSeq platform. We have now expanded this method to include two additional loci, HLA-DPB1 and DQB1....
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