High-throughput next-generation sequencing to genotype six classical HLA loci from 96 donors in a single MiSeq run.

doi:10.1111/tan.13133

Back to search

Article

High-throughput next-generation sequencing to genotype six classical HLA loci from 96 donors in a single MiSeq run.

HLA - 1 Nov 2017

Ehrenberg P K, Geretz A, Sindhu R K, Vayntrub T, Fernández Viña M A, Apps R, Michael N L, Thomas R

Abstract excerpt

Next generation sequencing (NGS) methods have been established as an efficient approach for HLA typing because unlike traditional Sanger sequencing, they provide unambiguous results at a reasonable cost. We previously developed a multi-locus index method to genotype four HLA loci (A, B, C, and DR...

Topics

Alleles
Genetic Loci
Genotype
HLA Antigens
High-Throughput Nucleotide Sequencing
Humans
Polymerase Chain Reaction
Tissue Donors

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.