Article
A multi-site study using high-resolution HLA genotyping by next generation sequencing.
Tissue antigens - 1 Mar 2011
Holcomb C L, Höglund B, Anderson M W, Blake L A, Böhme I, Egholm M, Ferriola D, Gabriel C, Gelber S E, Goodridge D, Hawbecker S, Klein R, Ladner M, Lind C, Monos D, Pando M J, Pröll J, Sayer D C, Schmitz-Agheguian G, Simen B B, Thiele B, Trachtenberg E A, Tyan D B, Wassmuth R, White S, Erlich H A
Abstract excerpt
The high degree of polymorphism at human leukocyte antigen (HLA) class I and class II loci makes high-resolution HLA typing challenging. Current typing methods, including Sanger sequencing, yield ambiguous typing results because of incomplete genomic coverage and inability to set phase for HLA allele determination. The 454 Life Sciences Genome Sequencer (GS FLX) next generation sequencing system coupled with...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.