Article
A multi-site study using high-resolution HLA genotyping by next generation sequencing.
Tissue antigens - 1 Mar 2011
Holcomb C L, Höglund B, Anderson M W, Blake L A, Böhme I, Egholm M, Ferriola D, Gabriel C, Gelber S E, Goodridge D, Hawbecker S, Klein R, Ladner M, Lind C, Monos D, Pando M J, Pröll J, Sayer D C, Schmitz-Agheguian G, Simen B B, Thiele B, Trachtenberg E A, Tyan D B, Wassmuth R, White S, Erlich H A
Abstract excerpt
The high degree of polymorphism at human leukocyte antigen (HLA) class I and class II loci makes high-resolution HLA typing challenging. Current typing methods, including Sanger sequencing, yield ambiguous typing results because of incomplete genomic coverage and inability to set phase for HLA al...
Topics
- Alleles
- Base Sequence
- Double-Blind Method
- Family Characteristics
- Genotype
- HLA Antigens
- High-Throughput Nucleotide Sequencing
- Humans
- Models, Biological
- Molecular Sequence Data