Article
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Scientific data - 1 Jan 2014
Cowley Glenn S, Weir Barbara A, Vazquez Francisca, Tamayo Pablo, Scott Justine A, Rusin Scott, East-Seletsky Alexandra, Ali Levi D, Gerath William Fj, Pantel Sarah E, Lizotte Patrick H, Jiang Guozhi, Hsiao Jessica, Tsherniak Aviad, Dwinell Elizabeth, Aoyama Simon, Okamoto Michael, Harrington William, Gelfand Ellen, Green Thomas M, Tomko Mark J, Gopal Shuba, Wong Terence C, Wong Terrence C, Li Hubo, Howell Sara, Stransky Nicolas, Liefeld Ted, Jang Dongkeun, Bistline Jonathan, Hill Meyers Barbara, Armstrong Scott A, Anderson Ken C, Stegmaier Kimberly, Reich Michael, Pellman David, Boehm Jesse S, Mesirov Jill P, Golub Todd R, Root David E, Hahn William C
Abstract excerpt
Using a genome-scale, lentivirally delivered shRNA library, we performed massively parallel pooled shRNA screens in 216 cancer cell lines to identify genes that are required for cell proliferation and/or viability. Cell line dependencies on 11,000 genes were interrogated by 5 shRNAs per gene. The proliferation effect of each shRNA in each cell line was assessed by transducing a population of 11M cells with one...
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