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Article

High-resolution, high-throughput HLA genotyping by next-generation sequencing.

Tissue antigens - 1 Nov 2009

Bentley G, Higuchi R, Hoglund B, Goodridge D, Sayer D, Trachtenberg E A, Erlich H A

Abstract excerpt

The human leukocyte antigen (HLA) class I and class II loci are the most polymorphic genes in the human genome. Hematopoietic stem cell transplantation requires allele-level HLA typing at multiple loci to select the best matched unrelated donors for recipient patients. In current methods for HLA typing, both alleles of a heterozygote are amplified and typed or sequenced simultaneously, often making it difficult...

Topics

Alleles
Base Sequence
Family Characteristics
Female
Gene Frequency
Genotype
HLA Antigens
High-Throughput Screening Assays
Histocompatibility Testing
Humans
Male

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Parents

Polymorphism, Genetic

Sequence Analysis, DNA

Severe Combined Immunodeficiency

Source

Open in PubMed Open full text

Identifiers

PMID: 19845894
DOI: 10.1111/j.1399-0039.2009.01345.x
PMCID: PMC4205125

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-19845894-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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