Article
Distributions of 11-loci HLA alleles typed by amplicon-based next-generation sequencing in South Koreans.
HLA - 1 Jun 2023
Baek In-Cheol, Choi Eun-Jeong, Kim Hyoung-Jae, Choi Haeyoun, Kim Tai-Gyu
Abstract excerpt
The range of HLA typing for successful hematopoietic stem cell transplantation (HSCT) is gradually expanding with the next-generation sequencing (NGS)-based improvement in its quality. However, it is influenced by the allocation of finances and laboratory conditions. HLA-A, -B, -C, -DRB1/3/4/5, -DQA1, -DQB1, -DPA1, and -DPB1 alleles were genotyped at the 3-field level by amplicon-based NGS using MiSeqDx system...
Topics
- Humans
- Alleles
- East Asian People
- Haplotypes
- HLA-B Antigens
- Histocompatibility Testing
- Multiplex Polymerase Chain Reaction
- HLA-A Antigens
- High-Throughput Nucleotide Sequencing
- Gene Frequency
