Article
HLA-DRB1, -DRB3, -DRB4 and -DRB5 genotyping at a super-high resolution level by long range PCR and high-throughput sequencing.
Tissue antigens - 1 Jan 2014
Ozaki Y, Suzuki S, Shigenari A, Okudaira Y, Kikkawa E, Oka A, Ota M, Mitsunaga S, Kulski J K, Inoko H, Shiina T
Abstract excerpt
Super high-resolution single molecule sequence-based typing (SS-SBT) is a human leukocyte antigen (HLA) DNA typing method to the field 4 level of allelic resolution (formerly known as eight-digit typing) to efficiently detect new and null alleles without phase ambiguity by combination of long ranged polymerase chain reaction (PCR) amplification and next-generation sequencing (NGS) technologies. We previously...
Topics
- Alleles
- DNA Primers
- Genotype
- HLA-DRB1 Chains
- HLA-DRB3 Chains
- HLA-DRB4 Chains
- HLA-DRB5 Chains
- High-Throughput Nucleotide Sequencing
- Histocompatibility Testing
- Humans
- Polymerase Chain Reaction
- Transplantation Immunology