Article
A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish.
Scientific reports - 6 Sept 2018
Shiraki Tomoya, Kawakami Koichi
Abstract excerpt
The CRISPR/Cas9 system can be introduced into zebrafish as transgenes. Namely, expression of single-guide RNA (sgRNA) and controlled expression of Cas9 in transgenic zebrafish enables the study of gene functions in specific cell types. This transgenic CRISPR/Cas9 approach would be more useful if multiple sgRNAs could be expressed simultaneously since we could knock-out a gene more efficiently or disrupt multiple...
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