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Article

Synthetic gene complementation to determine off-target silencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Kumar Dhirendra

Abstract excerpt

RNA interference (RNAi) is a conserved mechanism in a wide range of eukaryotes. Introduction of synthetic dsRNA could specifically target suppression of a gene or could result in off-target silencing of another gene due to sequence similarity. To verify if the observed phenotype in an RNAi transgenic line is due to silencing of a specific gene or if it is due to another nontarget gene, a synthetic gene...

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PMID: 25740373
DOI: 10.1007/978-1-4939-2453-0_21

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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