Article
Local gene silencing in plants via synthetic dsRNA and carrier peptide.
Plant biotechnology journal - 1 Oct 2014
Numata Keiji, Ohtani Misato, Yoshizumi Takeshi, Demura Taku, Kodama Yutaka
Abstract excerpt
Quick and facile transient RNA interference (RNAi) is one of the most valuable plant biotechnologies for analysing plant gene functions. To establish a novel double-strand RNA (dsRNA) delivery system for plants, we developed an ionic complex of synthetic dsRNA with a carrier peptide in which a cell-penetrating peptide is fused with a polycation sequence as a gene carrier. The dsRNA-peptide complex is 100-300 nm...
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