Article
Complementation of mutants in plant mitochondrial RNA editing by protoplast transfection.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Takenaka Mizuki, Zehrmann Anja
Abstract excerpt
A crucial and often decisive test of a nuclear gene being involved in a given process is the complementation of mutants. Restoring the wild type phenotype by the wild type gene introduced into the mutant is a major piece of evidence for the function of this gene. We have developed a rapid and reliable method to complement protoplasts from plants with mutations in mitochondrial RNA editing with the respective wild...
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