Vectors and parameters that enhance the efficacy of RNAi-mediated gene disruption in transgenic Drosophila.

Abstract excerpt

Whole-genome transgenic RNAi libraries permit systematic genetic screens in individual tissues of Drosophila. However, there is a high incidence of nonspecific phenotypes because of off-target effects. To minimize such effects, it is essential to obtain a deeper understanding of the specificity of action of RNAi. Here, in vivo assays are used to determine the minimum, contiguous nucleotide pairing required...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Animals, Genetically Modified
• Share to Cell Culture Techniques