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Article

Efficient and heritable functional knock-out of an adult phenotype in Drosophila using a GAL4-driven hairpin RNA incorporating a heterologous spacer.

Nucleic acids research - 15 Jun 2001

Piccin A, Salameh A, Benna C, Sandrelli F, Mazzotta G, Zordan M, Rosato E, Kyriacou C P, Costa R

Abstract excerpt

We have developed a modified RNA interference (RNAi) method for generating gene knock-outs in Drosophila melanogaster. We used the sequence of the yellow (y) locus to construct an inverted repeat that will form a double-stranded hairpin structure (y-IR) that is under the control of the upstream activating sequence (UAS) of the yeast transcriptional activator GAL4. Hairpins are extremely difficult to manipulate in...

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Identifiers

PMID: 11410678
DOI: 10.1093/nar/29.12.e55
PMCID: PMC55754

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Source receipt: pubmed, retrieved 7 Sept 2026.

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