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Article

Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA.

Proceedings of the National Academy of Sciences of the United States of America - 9 Dec 2014

Agrawal Anant A, McLaughlin Krystle J, Jenkins Jermaine L, Kielkopf Clara L

Abstract excerpt

Purine interruptions of polypyrimidine (Py) tract splice site signals contribute to human genetic diseases. The essential splicing factor U2AF(65) normally recognizes a Py tract consensus sequence preceding the major class of 3' splice sites. We found that neurofibromatosis- or retinitis pigmentosa-causing mutations in the 5' regions of Py tracts severely reduce U2AF(65) affinity. Conversely, we identified a...

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Identifiers

PMID: 25422459
DOI: 10.1073/pnas.1412743111
PMCID: PMC4267390

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA.PMID 25422459Read ↗

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