Article
The conserved RNA recognition motif 3 of U2 snRNA auxiliary factor (U2AF 65) is essential in vivo but dispensable for activity in vitro.
RNA (New York, N.Y.) - 1 Feb 2004
Banerjee Hiren, Rahn Andrew, Gawande Bharat, Guth Sabine, Valcarcel Juan, Singh Ravinder
Abstract excerpt
The general splicing factor U2AF(65) recognizes the polypyrimidine tract (Py tract) that precedes 3' splice sites and has three RNA recognition motifs (RRMs). The C-terminal RRM (RRM3), which is highly conserved, has been proposed to contribute to Py-tract binding and establish protein-protein contacts with splicing factors mBBP/SF1 and SAP155. Unexpectedly, we find that the human RRM3 domain is dispensable for...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.