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Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Dobrovolsky Vasily N, Cao Xuefei, Bhalli Javed A, Heflich Robert H

Abstract excerpt

The endogenous X-linked phosphatidyl inositol glycan class A gene (Pig-a) can be used as a reporter of in vivo somatic cell mutation in rats and mice. Pig-a mutant cells are deficient in specific protein surface markers and can be identified and quantified by immunofluorescent staining followed by high-throughput flow cytometry. Pig-a mutation detection is commonly performed with red blood cells (RBCs) because:...

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PMID: 31989564
DOI: 10.1007/978-1-0716-0223-2_18

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