Article
Extendable blocking probe in reverse transcription for analysis of RNA variants with superior selectivity.
Nucleic acids research - 1 Jan 2015
Ho Tho H, Dang Kien X, Lintula Susanna, Hotakainen Kristina, Feng Lin, Olkkonen Vesa M, Verschuren Emmy W, Tenkanen Tuomas, Haglund Caj, Kolho Kaija-Leena, Stenman Ulf-Hakan, Stenman Jakob
Abstract excerpt
Here we provide the first strategy to use a competitive Extendable Blocking Probe (ExBP) for allele-specific priming with superior selectivity at the stage of reverse transcription. In order to analyze highly similar RNA variants, a reverse-transcriptase primer whose sequence matches a specific variant selectively primes only that variant, whereas mismatch priming to the alternative variant is suppressed by...
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