Expanding the Design Rules for Discriminating Nucleic Acid Mutations via Mismatch-Exchange.

Abstract excerpt

Complementarity between nucleic acids via Watson-Crick base pairing formulates the basic principle for designing hybridization probes but often suffers low sequence selectivity against single nucleotide mutations. Herein, we report mismatch-exchange as a new design principle that allows the highly sensitive and robust discrimination of single nucleotide polymorphisms (SNPs) by simply manipulating the number and...

Topics

• Polymorphism, Single Nucleotide
• Base Pair Mismatch
• Humans
• Hepatitis B virus
• Mutation
• Nucleic Acid Hybridization