Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutant enrichment with 3'-modified oligonucleotides a practical PCR method for detecting trace mutant DNAs.

The Journal of molecular diagnostics : JMD - 1 Nov 2011

Lee Seung-Tae, Kim Ji-Youn, Kown Min-Jung, Kim Sun Wook, Chung Jae Hoon, Ahn Myung-Ju, Oh Young Lyun, Kim Jong-Won, Ki Chang-Seok

Abstract excerpt

Many clinical situations necessitate highly sensitive and reliable molecular assays; however, the achievement of such assays remains a challenge due to the inherent limitations of molecular testing methods. Here, we describe a simple and inexpensive enrichment technique that we call mutant enrichment with 3'-modified oligonucleotides (MEMO). The method is based on the use of a 3'-modified oligonucleotide primer...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA
Share to DNA Primers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genes, Neoplasm

Share to Molecular Diagnostic Techniques

Share to Nucleophosmin

Share to Oligonucleotides

Share to Proto-Oncogene Proteins B-raf

Share to Sensitivity and Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 21979247
DOI: 10.1016/j.jmoldx.2011.07.003
PMCID: PMC3194055

Genes

BRAF
EGFR
JAK2
KRAS
NPM1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutant enrichment with 3'-modified oligonucleotides a practical PCR method for detecting trace mutant DNAs.PMID 21979247Read ↗

Ribonucleotide-Modified Primer Overlapping Blocker Elongation (PROBE) Assay for Highly Specific Mutation Detection.Semantic proximity Read ↗

Ultraspecific and Amplification-Free Quantification of Mutant DNA by Single-Molecule Kinetic Fingerprinting.Semantic proximity Read ↗

COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing.Semantic proximity Read ↗

Modified Taq DNA Polymerase for Allele-Specific Ultra-Sensitive Detection of Genetic Variants.Semantic proximity Read ↗

Mutant Enrichment with 3'-Modified Oligonucleotides (MEMO)-Quantitative PCR for Detection of NPM1 Mutations in Acute Myeloid Leukemia.Semantic proximity Read ↗

Differential strand separation at critical temperature: a minimally disruptive enrichment method for low-abundance unknown DNA mutations.Semantic proximity Read ↗

A universal approach for mutation identification with a DNA probe-enzyme combination platform.Semantic proximity Read ↗

Extended Enrichment for Ultrasensitive Detection of Low-Frequency Mutations by Long Blocker Displacement Amplification.Semantic proximity Read ↗

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene.Semantic proximity Read ↗

Programmable One-Pot Enzymatic Reaction for Direct Fluorescence Detection of Ultralow-Abundance Mutations in the DNA Duplex.Semantic proximity Read ↗

Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing.Semantic proximity Read ↗

Site-specific insertion of endonuclease recognition sites into amplicons to improve post-PCR analysis sensitivity of gene mutation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.