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Article

Performance of amplicon-based next generation DNA sequencing for diagnostic gene mutation profiling in oncopathology.

Cellular oncology (Dordrecht, Netherlands) - 1 Oct 2014

Sie Daoud, Snijders Peter J F, Meijer Gerrit A, Doeleman Marije W, van Moorsel Marinda I H, van Essen Hendrik F, Eijk Paul P, Grünberg Katrien, van Grieken Nicole C T, Thunnissen Erik, Verheul Henk M, Smit Egbert F, Ylstra Bauke, Heideman Daniëlle A M

Abstract excerpt

PURPOSE: Next generation DNA sequencing (NGS) holds promise for diagnostic applications, yet implementation in routine molecular pathology practice requires performance evaluation on DNA derived from routine formalin-fixed paraffin-embedded (FFPE) tissue specimens. The current study presents a comprehensive analysis of TruSeq Amplicon Cancer Panel-based NGS using a MiSeq Personal sequencer (TSACP-MiSeq-NGS) for...

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Identifiers

PMID: 25209392
DOI: 10.1007/s13402-014-0196-2
PMCID: PMC13004472

Genes

BRAF
EGFR
KRAS
PIK3CA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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