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Article

Clinical validation of a next-generation sequencing screen for mutational hotspots in 46 cancer-related genes.

The Journal of molecular diagnostics : JMD - 1 Sept 2013

Singh Rajesh R, Patel Keyur P, Routbort Mark J, Reddy Neelima G, Barkoh Bedia A, Handal Brian, Kanagal-Shamanna Rashmi, Greaves Wesley O, Medeiros L Jeffrey, Aldape Kenneth D, Luthra Rajyalakshmi

Abstract excerpt

Transfer of next-generation sequencing technology to a Clinical Laboratory Improvement Amendments-certified laboratory requires vigorous validation. Herein, we validated a next-generation sequencing screen interrogating 740 mutational hotspots in 46 cancer-related genes using the Ion Torrent AmpliSeq cancer panel and Ion Torrent Personal Genome Machine (IT-PGM). Ten nanograms of FFPE DNA was used as template to...

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Share to High-Throughput Nucleotide Sequencing
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 23810757
DOI: 10.1016/j.jmoldx.2013.05.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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