Targeted Next Generation Sequencing as a Reliable Diagnostic Assay for the Detection of Somatic Mutations in Tumours Using Minimal DNA Amounts from Formalin Fixed Paraffin Embedded Material.

doi:10.1371/journal.pone.0149405

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Community

Back to search

Article

Targeted Next Generation Sequencing as a Reliable Diagnostic Assay for the Detection of Somatic Mutations in Tumours Using Minimal DNA Amounts from Formalin Fixed Paraffin Embedded Material.

PloS one - 1 Jan 2016

de Leng Wendy W J, Gadellaa-van Hooijdonk Christa G, Barendregt-Smouter Françoise A S, Koudijs Marco J, Nijman Ies, Hinrichs John W J, Cuppen Edwin, van Lieshout Stef, Loberg Robert D, de Jonge Maja, Voest Emile E, de Weger Roel A, Steeghs Neeltje, Langenberg Marlies H G, Sleijfer Stefan, Willems Stefan M, Lolkema Martijn P

Abstract excerpt

BACKGROUND: Targeted Next Generation Sequencing (NGS) offers a way to implement testing of multiple genetic aberrations in diagnostic pathology practice, which is necessary for personalized cancer treatment. However, no standards regarding input material have been defined. This study therefore aimed to determine the effect of the type of input material (e.g. formalin fixed paraffin embedded (FFPE) versus fresh...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA Mutational Analysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Formaldehyde

Share to High-Throughput Nucleotide Sequencing

Share to Mutation

Share to Neoplasms

Share to Paraffin Embedding

Share to Reproducibility of Results

Share to Tissue Fixation

Source

Open in PubMed Open full text

Identifiers

PMID: 26919633
DOI: 10.1371/journal.pone.0149405
PMCID: PMC4769293

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Targeted Next Generation Sequencing as a Reliable Diagnostic Assay for the Detection of Somatic Mutations in Tumours Using Minimal DNA Amounts from Formalin Fixed Paraffin Embedded Material.PMID 26919633Read ↗

Quality control material for the detection of somatic mutations in fixed clinical specimens by next-generation sequencing.Semantic proximity Read ↗

Validation of the Oncomine™ focus panel for next-generation sequencing of clinical tumour samples.Semantic proximity Read ↗

Targeted, high-depth, next-generation sequencing of cancer genes in formalin-fixed, paraffin-embedded and fine-needle aspiration tumor specimens.Semantic proximity Read ↗

Clinical validation of a next-generation sequencing screen for mutational hotspots in 46 cancer-related genes.Semantic proximity Read ↗

Routine use of the Ion Torrent AmpliSeq™ Cancer Hotspot Panel for identification of clinically actionable somatic mutations.Semantic proximity Read ↗

Comparative Analyses of Targeted Myeloid Cancer Next-Generation Sequencing Panel in Fresh Blood, Bone Marrow and FFPE Material.Semantic proximity Read ↗

Implementation of an ISO 15189 accredited next generation sequencing service for cell-free total nucleic acid (cfTNA) analysis to facilitate driver mutation reporting in blood: the experience of a clinical diagnostic laboratorySemantic proximity Read ↗

Validation of a next generation sequencing panel for detection of hotspot cancer mutations in a clinical laboratory.Semantic proximity Read ↗

Effective quality management practices in routine clinical next-generation sequencingSemantic proximity Read ↗

Comparison of Next-Generation Sequencing Panels and Platforms for Detection and Verification of Somatic Tumor Variants for Clinical Diagnostics.Semantic proximity Read ↗

Analysis of Pre-Analytic Factors Affecting the Success of Clinical Next-Generation Sequencing of Solid Organ MalignanciesSemantic proximity Read ↗

Versatile ion S5XL sequencer for targeted next generation sequencing of solid tumors in a clinical laboratory.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.