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Article

Clinical validation of KRAS, BRAF, and EGFR mutation detection using next-generation sequencing.

American journal of clinical pathology - 1 Jun 2014

Lin Ming-Tseh, Mosier Stacy L, Thiess Michele, Beierl Katie F, Debeljak Marija, Tseng Li-Hui, Chen Guoli, Yegnasubramanian Srinivasan, Ho Hao, Cope Leslie, Wheelan Sarah J, Gocke Christopher D, Eshleman James R

Abstract excerpt

OBJECTIVES: To validate next-generation sequencing (NGS) technology for clinical diagnosis and to determine appropriate read depth. METHODS: We validated the KRAS, BRAF, and EGFR genes within the Ion AmpliSeq Cancer Hotspot Panel using the Ion Torrent Personal Genome Machine (Life Technologies, Carlsbad, CA). RESULTS: We developed a statistical model to determine the read depth needed for a given percent tumor...

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Identifiers

PMID: 24838331
DOI: 10.1309/AJCPMWGWGO34EGOD
PMCID: PMC4332779

Genes

BRAF
EGFR
KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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