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Article

Clinical validation of KRAS, BRAF, and EGFR mutation detection using next-generation sequencing.

American journal of clinical pathology - 1 Jun 2014

Lin Ming-Tseh, Mosier Stacy L, Thiess Michele, Beierl Katie F, Debeljak Marija, Tseng Li-Hui, Chen Guoli, Yegnasubramanian Srinivasan, Ho Hao, Cope Leslie, Wheelan Sarah J, Gocke Christopher D, Eshleman James R

Abstract excerpt

OBJECTIVES: To validate next-generation sequencing (NGS) technology for clinical diagnosis and to determine appropriate read depth. METHODS: We validated the KRAS, BRAF, and EGFR genes within the Ion AmpliSeq Cancer Hotspot Panel using the Ion Torrent Personal Genome Machine (Life Technologies, C...

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Identifiers

PMID: 24838331
DOI: 10.1309/AJCPMWGWGO34EGOD
PMCID: PMC4332779

Genes

BRAF
EGFR
KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-24838331-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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