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Article

Validation and Application of a Custom-Designed Targeted Next-Generation Sequencing Panel for the Diagnostic Mutational Profiling of Solid Tumors.

PloS one - 1 Jan 2016

Froyen Guy, Broekmans An, Hillen Femke, Pat Karin, Achten Ruth, Mebis Jeroen, Rummens Jean-Luc, Willemse Johan, Maes Brigitte

Abstract excerpt

The inevitable switch from standard molecular methods to next-generation sequencing for the molecular profiling of tumors is challenging for most diagnostic laboratories. However, fixed validation criteria for diagnostic accreditation are not in place because of the great variability in methods and aims. Here, we describe the validation of a custom panel of hotspots in 24 genes for the detection of somatic...

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Identifiers

PMID: 27101000
DOI: 10.1371/journal.pone.0154038
PMCID: PMC4839685

Genes

APC
BRAF
EGFR
KRAS
NRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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