Efficient iterative CRISPR/Cas9 editing using sid-1 co-conversion and feeding RNAi in Caenorhabditis elegans.

Abstract excerpt

We present a sid-1 loss-of-function and restoration-of-function CRISPR/Cas9 co-conversion protocol in Caenorhabditis elegans. Introducing CRISPR reagents that induce sid-1 loss-of-function can produce survivors on lethal RNAi foods while reagents that induce sid-1 restoration-of-function can be screened for restoration of visible RNAi phenotypes. Both methods efficiently reduce the pool of candidates from...

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