Article
Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans.
PloS one - 1 Jan 2020
McDiarmid Troy A, Au Vinci, Moerman Donald G, Rankin Catharine H
Abstract excerpt
Improved genome engineering methods that enable automation of large and precise edits are essential for systematic investigations of genome function. We adapted peel-1 negative selection to an optimized Dual-Marker Selection (DMS) cassette protocol for CRISPR-Cas9 genome engineering in Caenorhabditis elegans and observed robust increases in multiple measures of efficiency that were consistent across injectors and...
Topics
- Alleles
- Animals
- CRISPR-Cas Systems
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Clustered Regularly Interspaced Short Palindromic Repeats
- Gene Editing
- Gene Targeting
- Homozygote
- Phenotype
- RNA, Guide, CRISPR-Cas Systems
- Toxins, Biological
- Transgenes