Article
Confounds of using the unc-58 selection marker highlights the importance of genotyping co-CRISPR genes.
PloS one - 1 Jan 2000
Rawsthorne-Manning Helena, Calahorro Fernando, G Izquierdo Patricia, Tardy Philippe, Boulin Thomas, Holden-Dye Lindy, O'Connor Vincent, Dillon James
Abstract excerpt
Multiple advances have been made to increase the efficiency of CRISPR/Cas9 editing using the model genetic organism Caenorhabditis elegans (C. elegans). Here we report on the use of co-CRISPR 'marker' genes: worms in which co-CRISPR events have occurred have overt, visible phenotypes which facilitates the selection of worms that harbour CRISPR events in the target gene. Mutation in the co-CRISPR gene is then...
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